Transplantation Immunology

Alloreactivity.- Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody
Binding and Mechanisms of Injury.- Cell Mediated Rejection.- Transplantation Tolerance.- Composite Tissue Transplantation.- Transplantation of the Sensitized Patient: Histocompatibility Testing.- Thoracic Organ Transplantation: Laboratory Methods.- HLA Typing by Sequence Specific Primers.- Human Leukocyte Antigen (HLA) Typing By DNA Sequencing.- Next-Generation HLA Sequencing using the 454 GS FLX System.- Chimerism Testing by Quantitative PCR  Using Indel Markers.- Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR).- Complement-dependent Cytotoxicity Crossmatch.- Lymphocyte Crossmatching by Flow Cytometry.- Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies.- C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens.- Tetramer Staining for the Detection of HLA-specific B cells.- A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood.- Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA).- Cylex  ImmuKnow Cell Function Assay.- Detection of Intracellular Cytokines.- Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients.- Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA.- RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing.