Microfluidic Techniques -

Microfluidic Techniques

Reviews and Protocols

Shelley D. Minteer (Herausgeber)

Buch | Hardcover
248 Seiten
2005
Humana Press Inc. (Verlag)
978-1-58829-517-0 (ISBN)
106,99 inkl. MwSt
Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Microfabrication Methods.- Overview of Advances in Microfluidics and Microfabrication.- to Microfabrication Techniques.- Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds.- Fabrication of Microelectrodes Using the Lift-Off Technique.- Laser Ablation as a Fabrication Technique for Microfluidic Devices.- Reversible, Room Temperature Bonding of Glass Devices for Microfluidics.- Biological Applications.- Solid-Phase Extraction in Packed Beds on Glass Microdevices.- Fabrication of Micromachined Magnetic Particle Separators for Bioseparation in Microfluidic Systems.- Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays.- Polymerase Chain Reaction in Miniaturized Systems:.- Polymerase Chain Reaction on Microchips.- A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes.- Microfluidic Ethanol Biobatteries on a Microchip.- Microfluidic Chambers for Cell Migration and Neuroscience Research.- Microtextured Polydimethylsiloxane Substrates for Culturing Mesenchymal Stem Cells.- Contraction Study of a Single Cardiac Muscle Cell in a Microfluidic Chip.- Microscale Integrated Sperm Sorter.

Erscheint lt. Verlag 15.11.2005
Reihe/Serie Methods in Molecular Biology ; 321
Zusatzinfo X, 248 p.
Verlagsort Totowa, NJ
Sprache englisch
Maße 155 x 235 mm
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete Pharmakologie / Pharmakotherapie
Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
Naturwissenschaften Biologie Zellbiologie
ISBN-10 1-58829-517-6 / 1588295176
ISBN-13 978-1-58829-517-0 / 9781588295170
Zustand Neuware
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