Neurodegeneration

Introduction to Neurodegenerative Diseases and Related Techniques.- In vivo and In vitro Determination of Cell Death Markers in Neurons.- Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods.- Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex.- Isolation and Culture of Postnatal Spinal Motoneurons.- Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration.- Neural Differentiation of Induced Pluripotent Stem Cells.- Developing Yeast Models of Human Neurodegenerative Disorders.- Modeling Dopamine Neuron Degeneration in C. elegans.- Role of the Proteasome in Fly Models of Neurodegeneration.- Modeling Neurodegenerative Diseases in Zebrafish Embryos.- Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases.- Modeling Focal Cerebral Ischemia In vivo.- Pharmacological Models of Parkison's Disease in Rodents.- Behavioral Phenotyping of Mouse Models of Neurodegeneration.- Characterization of Amyloid Deposits in Neurodegenerative Diseases.- Detection of Protein Aggregates in Neurodegenerative Diseases.- Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures.- Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium.- Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue.- Monitoring Mitophagy in Neuronal Cell Cultures.- Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging.- Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices.- Imaging Presynaptic Exocytosis in Corticostriatal Slices.- Microscopic Imaging of Intracellular Calcium in Live Cells using Lifetime based Ratiometric Measurements of Oregon Green BAPTA-1.- Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice.- Determination of Neurotransmitter Levels in Models of Parkinson’s Disease by HPLC-ECD.- Designing, Performing, and Interpreting a Microarray-based Gene Expression Study.- Adeno-associated Viral Gene Transfer for Neurodegenerative Diseases.- Specific Gene Silencing using RNAi in Cell Culture.- Stem Cell Transplantation for Spinal Cord Neurodegeneration.- Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases.