Neural Development

Isolation and Maintenance of Cortical Neural Progenitor Cells In Vitro.- Neural Induction with a Dopaminergic Phenotype from Human Pluripotent Stem Cells Through a Feeder-Free Floating Aggregation Culture.- Nucleoside Analog Labeling of Neural Stem Cells and Their Progeny.- Culture of Dissociated Hippocampal Neurons.- Culturing Mouse Cerebellar Granule Neurons.- Preparation of Primary Cultured Dopaminergic Neurons from Mouse Brain.- Culturing Astoryctes from Postnatal Rats.- Culturing Oligodendrocyte Lineage Cells from Neonatal Rats.- Isolation and Culture of Schwann Cells.- DNA Transfection: Calcium Phosphate Method.- The Gene-Gun Approach for Transfection and Labeling of Cells in Brain Slices.- Lentiviral Vector Production, Titration, and Transduction of Primary Neurons.- In-Ovo Electroporation in Embryonic Chick Spinal Cords.- Gene Transfer in Developing Chick Embryos: In Ovo Electroporation.- In Utero Electroporation in Mice.- General Introduction to In Situ Hybridization Protocol Using Non-Radioactively Labeled Probes to Detect mRNAs on Tissue Sections.- Immunofluorescence Staining with Frozen Mouse or Chick Embryonic Tissue Sections.- β-Galactosidase Staining of LacZ Fusion Proteins in Whole Tissue Preparations.- Chromatin Immunoprecipitation Assay of Brain Tissues Using Percoll Gradient-Purified Nuclei.- In Vivo Dual Luciferase Reporter Assay with Chick Neural Tube In Ovo Electroporation System.- Growth Cone Collapse Assay.- The Stripe Assay: Studying Growth Preference and Axon Guidance on Binary Choice Substrates In Vitro.- Microcontact Printing of Substrate-Bound Protein Patterns for Cell- and Tissue Culture.- Semi-Automated Analysis of Dendrite Morphology in Cell Culture.- Monitoring Synaptic Plasticity by Imaging AMPA Receptor Content and Dynamics on Dendritic Spines.- MorphologicalAnalysis of Neuromuscular Junctions by Immunofluorescent Staining of Whole-Mount Mouse Diaphragms- Routine Histology Techniques for the Developing and Adult Central Nervous System.- Cryosectioning.- The Golgi-Cox Method.- Neuroanatomical Tract-Tracing Using Biotinylated Dextran Amine.- Retrograde Tracing Technique for Neonatal Animals.