Plant-Pathogen Interactions

Galaxy as a Platform for Identifying Candidate Pathogen Effectors.- Bioinformatic analysis of expression data to identify effector candidates.- Two-dimensional data binning for the analysis of genome architecture in filamentous plant pathogens and other eukaryotes.- On The Statistics Of Identifying Candidate Pathogen Effectors.- High-throughput imaging of plant immune responses.- In vivo protein-protein interaction studies with BiFC: conditions, cautions and caveats.- Particle bombardment-mediated transient expression to identify localization signals in plant disease resistance proteins and target sites for the proteolytic activity of pathogen effectors.- Purification of fungal haustoria from infected plant tissue by flow cytometry.- Functional Characterisation of Nematode Effectors in Plants.- Silencing of aphid genes by feeding on stable transgenic Arabidopsis thaliana.- Leaf-disc assay based on transient over-expression in Nicotiana benthamiana to allow functional screening of candidate effectors from aphids.- A growth quantification assay for Hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana.- Simple quantification of in planta fungal biomass.- Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum.- DIGE-ABPP by click chemistry: Pairwise comparison of serine hydrolase activities from the apoplast of infected plants.- A Simple and Fast Protocol for the Protein Complex Immunoprecipitation (Co-IP) of Effector – Host Protein Complexes.- An Arabidopsis and Tomato Mesophyll Protoplast System for fast Identification of early MAMP-triggered Immunity-Suppressing Effectors.- Production of RXLR effector proteins for structural analysis by X-ray crystallography.- The Do’s and Don’ts of effectoromics.- Protoplast cell death assay to study Magnaporthe oryzae AVR gene function in rice.- A Bacterial Type III Secretion Based Delivery System for Functional Assays of Fungal Effectors in Cereals.-Capture arrays to annotate resistance genes in plant genomes and to accelerate plant resistance gene discovery.