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Salmonella

Methods and Protocols

Heide Schatten, Abraham Eisenstark (Herausgeber)

Buch | Hardcover

301 Seiten

2014 | 2nd ed. 2015
Humana Press Inc. (Verlag)
978-1-4939-1624-5 (ISBN)

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Salmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research. With new chapters on molecular assays for detection, identification and serotyping of salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, development of bacterial nanoparticle vaccine, attachment of nanoparticle cargo to biotinylated salmonella for combination bacteriotherapy against tumors, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Salmonella: Methods and Protocols, Second Edition present methods that are of value to investigators in the salmonella field.

Luminex® Multiplex Bead Suspension Arrays for the Detection and Serotyping of Salmonella spp..- Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle.- Determination of Antimicrobial Resistance in Salmonella spp..- Red-mediated Recombineering of Salmonella enterica Genomes.- A Method to Introduce an Internal Tag Sequence into a Salmonella Chromosomal Gene.- Generation and use of Site-directed Chromosomal cyaA’ Translational Fusions in Salmonella enteric.- Detection of Antimicrobial (Poly)Peptides with Acid Urea Polyacrylamide Gel Electrophoresis followed by Western Immunoblot.- Detecting non-typhoid Salmonella in Humans by Enzyme-linked Immunosorbent Assays (ELISAs): Practical and Epidemiological Aspects.- Study of the Stn Protein in Salmonella; A Regulator of Membrane Composition and Integrity.- Development of a Bacterial Nanoparticle Vaccine.- Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium Membranes Designed for Combination Bacteriotherapy Against Tumors.- Applications of Microscopy in Salmonella Research.- Live Cell Imaging of Intracellular Salmonella enteric.- In vitro Modeling of Gallbladder-associated Salmonella spp. Colonization.- Salmonella Phages and Prophages – Genomics, Taxonomy, and Applied Aspects.

Reihe/Serie	Methods in Molecular Biology ; 1225
Zusatzinfo	26 Illustrations, color; 26 Illustrations, black and white; XI, 301 p. 52 illus., 26 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Antimicrobial Resistance • bacterial nanoparticle vaccine • bacteriotherapy against tumors • biotinylated Salmonella • chromosomal gene analysis • host cells • in vitro modeling of gallbladder • Mutagenesis • nanoparticle cargo • phages and prophages • Salmonella • serotyping
ISBN-10	1-4939-1624-6 / 1493916246
ISBN-13	978-1-4939-1624-5 / 9781493916245
Zustand	Neuware