Rab GTPases

Guangpu Li, Ph.D. Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences Center 975 NE 10th Street, BRC 417B Oklahoma City, OK 73104 Tel: (405) 271-2227 ext. 61232 Fax: (405) 271-3910 E-mail: guangpu-li@ouhsc.edu

Rab Family of GTPases.- Bioinformatic Approaches to Identifying and Classifying Rab Proteins.- Rab-NANOPS: FRET-Biosensors for Rab Membrane Nano clustering and Prenylation Detection in Mammalian Cells.- High Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins.- Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts.- Analysis of the Interactions Between Rab GTPases and Class V Myosins.- Assaying the Interaction of the Rab Guanine Nucleotide Exchange Protein Sec2 with the Upstream Rab, a Downstream Effector and a Phosphoinositide.- Kinetic Activation of Rab8 Guanine Nucleotide Exchange Factor Rabin8 by Rab11.- Ypt1 and TRAPP Interactions: Optimization of Multi-Color Bimolecular Fluorescence Complementation in Yeast.- Identifying a Rab Effector on the Macro autophagy Pathway.- Functional Analysis of Rab27 and its Effector Slp-2a in Renal Epithelial Cells.- Small GTPases in Acrosomal Exocytosis.- Rab Antibody Characterization: Comparison of Rab14 Antibodies.- Selective Visualization of GLUT4 Storage Vesicles and Associated Rab Proteins Using IRAP-pHluorin.- 3D Time-Lapse Analysis of Rab11/FIP5 Complex: Spatiotemporal Dynamics During Apical Lumen Formation.- In Vitro and In Vivo Characterization of the Rab11-GAP Activity of Drosophila Evi5.- Characterization of the Role Rab25 in Energy Metabolism and Cancer Using Extracellular Flux Analysis and Material Balance.- Measurement of Rab35 Activity by the GTP-Rab35 Trapper RBD35.- Analysis of Connecdenn 1-3 (DENN1A-C) GEF Activity for Rab35.- Assay of Rab17 and its Guanine Nucleotide Exchange Factor Rabex-5 in Dendrites of Hippocampal Neurons.- Methods for Analysis of AP-3/Rabin4’ in Regulation of Lysosome Distribution.- Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay.- Identification of the Rab5 Binding Site in p110b - Assays for PI3Kb Binding to Rab5.- Role of the Rab5 Guanine Nucleotide Exchange Factor,Rme-6, in the Regulation of Clathrin-Coated Vesicle Uncoating.- Differential Effects of Overexpression of Rab5 and Rab22 on Autophagy in PC12 Cells With or Without NGF.- Determining the Role of Rab7 in Constitutive and Ligand-Mediated Epidermal Growth Factor Receptor Endocytic Trafficking Using Single Cell Assays.- Visualizing Directional Rab7 and TrkA Co-Trafficking in Axons by pTIRF Microscopy.- Quantitative Bead-Based Flow Cytometry for Assaying Rab7 GTPase Interaction with the Rab-Interacting Lysosomal Protein (RILP) Effector Protein.