Protein-Carbohydrate Interactions

A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases.- Quantitative Kinetic Characterization of Glycoside Hydrolases using High-Performance Anion-Exchange Chromatography (HPAEC).- Measuring Enzyme Kinetics of Glycoside Hydrolases using the 3,5-Dinitrosalicylic Acid Assay.- An Improved Kinetic Assay for the Characterization of Metal-Dependant Pectate Lyases.- Colorimetric Detection of Acetyl Xylan Esterase Activities.- Methods for Determining Glycosyltransferase Kinetics.- Analyzing Activities of Lytic Polysaccharide Monooxygenases by Liquid Chromatography and Mass Spectrometry.- Carbohydrate Depolymerization by Intricate Cellulosomal Systems.- Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions.- Quantifying CBM Carbohydrate Interactions using Microscale Thermophoresis.- Characterization of Protein-Carbohydrate Interactions by NMR Spectroscopy.- Measuring the Biomechanical Loosening Action of Bacterial Expansins on Paper and Plant Cell Walls.- Bioinspired Assemblies of Plant Cell Walls for Measuring Protein-Carbohydrate Interactions by FRAP.- CBMs as Probes to Explore Plant Cell Wall Heterogeneity using Immunocytochemistry.- Determining the Localization of Carbohydrate Active Enzymes within Subcellular Compartments of Gram-Negative Bacteria.- Analysis of Complex Carbohydrate Composition in Plant Cell Wall using Fourier Transformed Mid-Infrared Spectroscopy (FT-IR).- Separation and Visualization of Glycans by Fluorophore-Assisted Carbohydrate Electrophoresis.- A Rapid Procedure for the Purification of 8-Aminopyrene Trisulfonate (APTS)-Labelled Glycans for Capillary Electrophoresis (CE)-Based Enzyme Assays.- Probing the Complex Architecture of Multimodular Carbohydrate-Active Enzymes using a Combination of Small Angle X-Ray Scattering and X-Ray Crystallography.- Metagenomics and CAZyme Discovery.- Identification of Genes Involved in the Degradation of Lignocellulose using Comparative Transcriptomics.- Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies using Mass Spectrometry.