Production of Crude Cellulase Enzyme from Soil Fungi

Enzymes production is a growing field of biotechnology. Cellulase enzymes are one among of the hydrolytic enzymes capable of hydrolyzing the most abundant organic polymer i.e. "cellulosic material" to produce the smaller sugar components i.e. "glucose, cellobiose and oligomers. The soil fungi have been the best effective microorganisms for cellulase production under solid state fermentation (SSF) technology. Crude cellulase is a group of enzyme consisting of three different of enzymes: endo-1, 4- beta-D glucanase , exo-1,4- beta-D glucanase)and beta- glucosidase. This study involves isolation, identification of some soil fungi and production of crude cellulose enzymes from them by using wheat bran as substrate under solid state fermentation (SSF). The enzyme activity for the different isolated enzymes produced is studied and also study the application of produced enzymes in bioconversion of OOMSW biomass as substrate.