Manual of Sperm Retrieval and Preparation in Human Assisted Reproduction

Ashok Agarwal is the Director of the Clinical Andrology Laboratory and Reproductive Tissue Bank and the Director of Research at the American Center for Reproductive Medicine. He holds these positions at The Cleveland Clinic Foundation, where he is a Professor at the Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio, USA. Ahmad Majzoub is a Consultant in the Department of Urology at the Hamad Medical Corporation, Doha and an Assistant Professor of Clinical Urology at Weill Cornell Medicine, Doha, Qatar. Sandro C. Esteves is Medical Director, ANDROFERT Andrology & Human Reproduction Clinic, Campinas, and a Professor of Urology at the University of Campinas (UNICAMP), Campinas, Brazil.

Part I. Introduction: 1. Anatomy and physiology of the male reproductive system Ryan Scribner and Nick N. Tadros; 2. Evaluation of candidates for sperm retrieval Darren J. Bryk, Neel V. Parekh and Sarah C. Vij; 3. Testicular histopathology and the role of testis biopsy Gianmartin Cito; Part II. Sperm Retrieval: 4. History of surgical sperm retrieval techniques Rupin Shah; 5. Epididymal sperm retrieval: indications, surgical protocol, and outcomes Jason P. Akerman and R. Matthew Coward; 6. Testicular sperm retrieval: indications, surgical protocol, and outcomes Ahmad Majzoub, Sandro C. Esteves and Chak-Lam Cho; 7. Surgical and non-surgical sperm retrieval techniques in patients with ejaculatory dysfunctions Mohamed Adnan, Omer Raheem and Laith M. Alzweri; 8. Sperm retrieval in non-azoospermic men: indications, protocol, and outcomes Sandro C. Esteves and Ahmad Majzoub; 9. Predictors of positive surgical sperm retrieval in azoospermic males: evaluation of clinical, laboratory, and histopathologic factors Kareim Khalafalla, Ahmed H. Almalki, Ahmad Majzoub and Sandro C. Esteves; 10. Methods for enhancing surgical sperm retrieval success rate Jordan Cohen and Ranjith Ramasamy; Part III. Laboratory Handling of Retrieved Sperm: 11. Critical factors for optimizing sperm handling and ICSI outcomes Edson Borges, Amanda Souza Setti and Daniela Paes de Almeida Ferreira Braga; 12. Sperm cryopreservation Rakesh Sharma, Kruyanshi Master and Ashok Agarwal; 13. Future directions in sperm handling for ART Manesh Panner Selvam Kumar, Catalina Barbarosie and Ashok Agarwal.