Mitochondria

A Guide to Computational Methods for Predicting Mitochondrial Localization.- Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues.- Isolation of Mitochondria From Saccharomyces Cerevisiae.- Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes.- Isolation Of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue.- Label-Free Quantitative Analysis of Mitochondrial Proteomes using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and ‘Total Protein Approach’.- Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry.- Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells.- Chemical Crosslinking in Intact Mitochondria.- Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression.- Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast.- Localizing Mrnas Encoding Mitochondrial Proteins in Yeast byFluorescence Microscopy and Subcellular Fractionation.- Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues using Seahorse XF96 Analyzer.- Application of FRET-Based Biosensor “Ateam” for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells.- A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake.- New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis Elegans.- Single Molecule Tracking and Localization Of Mitochondrial Protein Complexes in Live Cells.- Analysis of Yeast Mitochondria by Electron Microscopy.- Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-Tomography.- Assays for Mitophagy in Yeast.- Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis Elegans.- Assessing Mitochondrial Unfolded Protein Response in Mammalian Cells.- Analysis of Mitochondrial RNA-Processing Defects in Patient-Derived Tissues by Qrt-PCR and Rnaseq.-Identification of Disease-Causing Mutations by Functional Complementation of Patient-Derived Fibroblast Cell Lines.