Functional Microbial Genomics

Edited by Professor Brendan Wren and Nick Dorrell

GENOME SEQUENCE ANALYSIS; Annotation of genomes ; From sequence to consequence: in silico hypothesis generation and testing; The atlas visualisation of genome-wide information; CONSTRUCTION OF DNA MICROARRAYS; Microarray design for bacterial genomes; Glass slides microarrays for bacterial genomes; COMPARATIVE NUCLEIC ACID ANALYSIS ; Representational display analysis of Cdna and genome comparisons; Application of DNA microarrays for comparative and evolutionary genomics; Gene expression during host-pathogen interactions: approaches to bacterial mRNA extraction and labelling for microarray analysis; High throughput in vivo screens: signature-tagged mutagenesis; Further strategies for signature-tagged mutagenesis and the application of oligonucleotide microarrays for the quantitation of DNA-tagged strains; PROTEOME ANALYSIS; Advances in bacterial proteome analysis; Discovery of protein-protein interaction using two-hybrid systems; SECTION V APPLICATIONS OF MICROBIAL GENOMICS ; Cloning the metagenome: Culture-independent access to the diversity and functions of the uncultivated microbial world; Reverse vaccinology: from genome to vaccine; Microbial genomics for antibiotic target discovery ; CASE STUDIES - BACTERIA; Helicobacter pylori functional genomics; Streptomyces coelicolor A3(2): from genome sequence to function; Functional analysis of the Bacillus subtilis genome; SECTION VII CASE STUDIES - PARASITES; Plasmodium falciparum DNA microarrays and interpretation of data; Functional analysis of the Plasmodium falciparum genome using transfection; Chromosome fragmentation as an approach to whole genome analysis in trypanosomes