Basic Methods in Antibody Production and Characterization

Gary C. Howard, Delia R. Bethell (Herausgeber)

Buch | Softcover

284 Seiten

2021
CRC Press (Verlag)
978-0-367-20244-6 (ISBN)

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First published in 2001: Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies.
Antibodies described include polyclonal and monoclonal and those made by the phage display. Basic Methods in Antibody Production and Characterization focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications.
Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications. Ample references arm researchers with many applications pertaining to the particular technology.

Gary C. Howard, Ph.D., is a senior scienfitic editor for the J. David Gladstone Institutes. Delia R. Bethell, Ph.D., is director of research with BioSeparations, Inc., in Tuscon, AZ.

1. Starting Out

Delia R. Bethell and Gary C. Howard

2. Animal Welfare And Regulatory Issues

Sigmund T. Rich

3. Antigens

Donald H. Lewis

4. Adjuvants

Jory R. Baldridge and Michael J. Lacy

5. Polyclonal Antibodies

Eric S. Bean

6. Monoclonal Antibody Production

Sandy J. Stewart

7. Screening Techniques

Gamal E. Osman and Warren E. Ladiges

8. Antibody Production By Ascites Growth

Kristi R. Harkins

9. Growth Of Cell Lines In Bioreactors

Beverly J. Norris, Michael J. Gramer, and Mark Hirschel

10. Phage Display

Peter Amersdorfer and James D. Marks

11. Antibody Purification Methods

Kristi R. Harkins

12. Molecular Characterization Of Immunoglobulin Genes

Warren Ladiges and Gamal E. Osman

13. Antibody Storage

Kathryn Elwell and Delia R. Bethell

14. Chemically Modifying Antibodies

Kathryn Elwell and Gary C. Howard

15. Western Blotting

Lee Bendickson and Marit Nilsen-Hamilton

16. Immunohistochemical Methods

Kendrick J. Morrell, Neil M. Hand, and Nicholas R. Griffin

Erscheinungsdatum	24.09.2020
Reihe/Serie	Routledge Revivals
Zusatzinfo	1 equations; 8 Tables, black and white
Verlagsort	London
Sprache	englisch
Maße	156 x 234 mm
Gewicht	408 g
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie
	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
	Naturwissenschaften ► Biologie
ISBN-10	0-367-20244-1 / 0367202441
ISBN-13	978-0-367-20244-6 / 9780367202446
Zustand	Neuware