Handbook of Translational Transcriptomics

Anton Buzdin is professor and head of laboratories at Sechenov First Moscow Medical University, Moscow Institute of Physics and Technology, and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry. He was elected as the chair of Biostatistics and Bioinformatics subgroup, Pathobiology Group of European Organization for Research and Treatment of Cancer (EORTC). Professor Buzdin is cofounder and chief scientific officer of Oncobox, which is a translational oncology company. He has authored more than 170 scientific papers and patent applications, and has worked as chief executive of several biotechnological and biomedical companies in Russia, Hong Kong and in the United States. He has also chaired two clinical trials of transcriptomics-based diagnostic tools in oncology. Professor Buzdin’s team pioneered bioinformatic quantitative analysis of molecular pathways using gene expression data and developed specialized algorithms and software packages to personalize prescription of targeted immunotherapy and chemotherapy drugs to cancer patients that were clinically validated and commercialized.

1. Past, current and future of transcriptomics 2. Pitfalls of transcriptomics, and selection of the most appropriate transcriptomic technique 3. RNA sequencing in wet lab 4. Expression arrays in wet lab 5. High-throughput and multiplex PCR in wet lab to measure gene expression 6. Processing primary gene expression data: normalization, harmonization and data QC 7. Data check and comparison with the expression databases 8. Finding differentially expressed genes and gene sets 9. Molecular pathway analysis using transcriptomic data 10. OMICSwise analysis of RNA splicing 11. Transcriptomic biomarkers in medicine 12. Translational transcriptomics for personalized oncology 13. Transcriptomics for modern biotechnology 14. Transcriptomics and quantitative proteomics: competition or symbiosis?