Handbook of DNA Profiling

Dr. Hirak Ranjan Dash completed his Ph.D. from the Department of Life Science, National Institute of Technology, Rourkela, India, and is currently working as a DNA fingerprinting expert at the Forensic Science Laboratory, Bhopal, Madhya Pradesh, India. He received his M.Sc. in Microbiology from Orissa University of Agriculture and Technology, Odisha, India. His research interests include forensic microbiology, thanatomicrobiome analysis, molecular microbiology, DNA fingerprinting and genetic manipulation. He has written 4 books and published 29 research papers, 14 book chapters, 12 conference proceedings, and 4 popular science articles. Dr. Pankaj Shrivastava received his Ph.D. in Microbiology with a specialization in Biotechnology from the Department of Biological Science, Rani Durgawati University, Jabalpur, Madhya Pradesh, India. He is working as In charge of the Forensic DNA Fingerprinting facility of Govt. of Madhya Pradesh, India at Sagar. He has more than 13 years of experience as the bench worker for examining and reporting a wide range of criminal cases using DNA technology and deposing the court evidence. His research interests include population DNA database, improvement of methods in forensic DNA typing, finding rapid protocols for the technology, and microbial forensics. He is the recipient of the prestigious Pt. Govind Vallabh Pant Award from the Bureau of Police Research and Development, Ministry of Home Affairs, Govt. of India twice, and Anusrajan Award from AIISECT University, Bhopal for his authored books. He is also a recipient of the FICCI Smart Policing award for developing a fast DNA typing protocol. He is visiting faculty of many universities, Police training Institutes, and Judicial officers training Institutes. He has written 13 books and published 65 research papers in the journals of repute in forensic science, 21 book chapters, 84 conference proceedings, 15 popular science articles. Jose A. Lorente is a Professor of Forensic Medicine at the University Of Granada, Spain. After graduating at the Faculty of Medicine of Granada in 1985, he received Ph.D. Honors in Medicine and Surgery at the University of Granada, in 1989. Dr. Lorente has published over 140 peer-reviewed papers and several books and book chapters. He has a special focus on the use of DNA and its application to human rights (he launched the first-ever database to identify missing people, the Spanish Phoenix Program, back in 1999); he also created and launched the DNA-PROKIDS Program in 2004. His areas of interest in forensics also deal with population variability and analysis of old and ancient DNA samples and databases expansion and control. Dr. Lorente is also actively working in medical genomics and he's the scientific director of the Center for Genomics and Oncological Research (GENYO), where his team focuses on liquid biopsy and cancer interception. Dr. Lorente is the founder and first president of the AICEF (Ibero-Latin American Network of Forensic Sciences), and an honorary member of the AFSN (Asian Forensic Sciences Network).

1. Introduction to forensic DNA typing



2. Biological sources of DNA: the target materials for forensic DNA analysis



3. Collection, preservation and transportation of DNA evidences



4. Tools and techniques used in forensic DNA typing



5. Forensic DNA fingerprinting: evaluation of the currently used autosomal STR markers and kits



6. Forensic DNA fingerprinting: evaluation of the currently used gonosomal markers and kits



7. Usefulness of SNPs in forensic DNA analysis



8. DNA databases: A boon to forensic DNA analysis



9. Statistical evaluation of forensic DNA evidence



10. Potential of DNA technique based body fluid identification



11. DNA fingerprinting and legislation



12. A glimpse of famous cases in history solved by DNA typing



13. Usefulness of autosomal STR analysis in solving cases: a case study



14. A case study on the usefulness of Y-STR typing technique



15. Usefulness of X-STR analysis in solving cases: a case study



16. Usefulness of mitochondrial DNA analysis in solving cases: a case study



17. Application of NGS in solving mysterious cases



18. A case study on disputed paternity solved by DNA typing



19. Identification of mutilated bodies: a case study



20. A case study on murder mystery solved by DNA typing



21. Identification of sexual assault culprit by DNA typing: a case study



22. Disaster victim identification by DNA typing



23. Cases of robbery solved by DNA typing



24. Ancient DNA analysis: A case study



25. Miscellaneous cases solved by DNA typing



26. DNA analysis for prenatal diagnosis



27. Analysis of DNA for diagnosis of neonatal diseases



28. Diseases of skin, hair and nail and their analysis through DNA



29. A Paediatrician's perspective of DNA analysis



30. Diseases of metabolism and endocrine disorder by DNA analysis



31. DNA analysis in malignancies; diagnostic and prognostic value



32. The role of DNA analysis in drug reactions



33. Detection of various diseases through DNA typing: case studies



Section V: Modifications in routine methodologies to solve challenging cases



34. Modification of the manual DNA extraction protocols to obtain results in a case



35. A modified protocol of automated DNA extraction technique to obtain results



36. Modification of PCR conditions to obtain results in a case



37. Modification of various parameters of capillary electrophoresis to obtain results in a case



38. Required modifications of default software parameters to obtain results in a case



39. Application of various statistical tools to draw conclusion in a case



40. Cases pertaining to wild-life crimes solved by DNA typing



41. Usefulness of plant DNA typing in solving different cases



42. Microbial typing to solve various cases



43. Touch DNA analysis



44. RAPID DNA analysis



45. Interpretation of mixed profiles



46. Role of population data in strengthening forensic DNA analysis



47. Role of genealogy databases for identification of suspected perpetrators



48. Familial searching: an application of forensic DNA profiling



49. An insight on miscarriages of justice through DNA typing



50. Validation kits and instruments and its necessity



51. Quality control in forensic DNA analysis



52. DNA phenotyping: the technique of future



53. Recommendations on report writing of DNA profiling results



54. The role of scientific societies in generating best practices in forensic DNA profiling