Biotechnology

Rolf Schmid studied chemistry, biochemistry and microbiology in Munich and Freiburg (Germany). After postdoctoral training in Gif-sur-Yvette (France) and Austin (USA), he joined Henkel KGaA in Dusseldorf (Germany) where he worked for 15 years, eventually as Director R&D Biotechnology. In 1987, he was invited to direct the Institute for Enzyme Technology at Braunschweig Technical University and the Division of Enzyme Technology and Chemistry of Natural Products at the 'Gesellschaft für biotechnologische Forschung' (GBF) in Braunschweig (Germany). From 1993 to 2009, he was Director of the Institute for Enzyme Technology at Stuttgart University (Germany). After having obtained an MBA diploma from the University of Reutlingen in 2007, he founded the consulting agency www.bio4business.eu in Stuttgart, followed in 2015 by the Steinbeis Consulting Agency www.asia-tech.eu. Dr. Claudia Schmidt-Dannert is Distinguished McKnight Professor in the Department of Biochemistry, Molecular Biology and Biophysics, at the University of Minnesota (USA). She studied Biology and Biochemistry at the Carolo Wilhelmina University Braunschweig (Germany), where she received her Ph.D. in Biochemistry in 1994. Schmidt-Dannert held a Postdoc position in Molecular Biotechnology at the University of Stuttgart (Germany) and became Professor at the University of Minnesota in 2010. Her research focuses on exploring and utilizing the metabolic machineries of plants and microorganisms to enable the discovery and synthesis of valuable microbial or plant-derived compounds in engineered microbial cells.

INTRODUCTION
Early developments
Biotechnology today
MICROBIOLOGY
Viruses
Bacteriophages
Microorganisms
Bacteria
Yeasts
Fungi
Algae
Some bacteria of importance for biotechnology
Microorganisms: isolation, preservation, safety
Microorganisms: strain improvement
BIOCHEMISTRY
Biochemistry
Amino acids, peptides, proteins
Enzymes: structure, function, kinetics
Sugars, glycosides, oligo- and polysaccharides
Lipids, membranes, membrane proteins
Metabolism
GENETIC ENGINEERING
DNA: structure
DNA: function
RNA
Genetic engineering: general steps
Preparation of DNA
Other useful enzymes for DNA manipulation
PCR: general method
PCR: laboratory methods
DNA: synthesis and size determination
DNA sequencing
Transfer of foreign DNA in living cells (transformation)
Gene cloning and identification
Gene expression
Gene silencing
Epigenetics
Gene libraries and gene mapping
Genetic maps of prokaryotes
Genetic maps of eukaryotes
Metagenomics
CELL BIOLOGY
Cell biology
Stem cells
Blood cells and immune system
Antibodies
Reporter groups
BIOENGINEERING
Solid state fermentation (SSF)
Growing microorganisms
Growth kinetics and product formation
Fed-batch, continuous and high cell density fermentation
Fermentation technology
Fermentation technology: scale-up
Cultivation of mammalian cells
Mammalian cell bioreactors
Enzyme and cell reactors
Recovery of bioproducts
Recovery of proteins: chromatography
Economic aspects of industrial processes
FOOD AND FOOD ADDITIVES
Alcoholic beverages
Beer
Fermented food
Food and lactic acid fermentation
Prebiotics and probiotics
Baker's yeast and fodder yeasts
Fodder yeasts from petroleum feedstocks, single cell oil
Amino acids
L-Glutamic acid
D,L-Methionine, L-lysine, and L-threonine
AspartameTM, L-phenylalanine, and L-aspartic acid
Amino acids via enzymatic transformation
Vitamines
Nucleosides and nucleotides
INDUSTRIAL PRODUCTS
Bio-Ethanol
1-Butanol
Higher alcohols and alkenes
Acetic acid/ vinegar
Citric acid
Lactic acid, 3-hydroxy-propionic acid (3-HP)
Gluconic acid and "green" sugar chemicals
Dicarboxylic acids
Biopolymers: Polyesters
Biopolymers: Polyamides
Polysaccharides
Biosurfactants
Fatty Acids and their esters
ENZYME TECHNOLOGY
Biotransformation
Technical enzymes
Applied enzyme catalysis
Regio- and enantioselective enzymatic synthesis
Enzymes as processing aids
Detergent enzymes
Enzymes for starch hydrolysis
Enzymatic starch hydrolysis
Enzymes and sweeteners
Enzymes for the hydrolysis of cellulose and polyoses
Enzymes in pulp and paper processing
Pectinases
Enzymes and milk products
Enzymes in baking and meat processing
Other enzymes for food products and animal feed
Enzymes in leather and textile treatment
Procedures for obtaining novel technical enzymes
Protein design
ANTIBIOTICS
Antibiotics: occurrence, applications, mechanism of action
Antibiotics: screening, industrial production, and mechanism of action
Antibiotic resistance
ß-Lactam antibiotics: structure, biosynthesis, and mechanism of action
ß-Lactam antibiotics: manufacture
Amino acids and peptide antibiotics
Glycopeptide, lipopeptide, polyether, and nucleoside antibiotics
Aminoglycoside antibiotics
Tetracyclines, chinones, chinolones, and other aromatic antibiotics
Macolide antibiotics
New pathways to antibiotics
PHARMACEUTICALS AND MEDICAL TECHNOLOGY
Insulin
Growth hormone and other hormones
Hemoglobin, serum albumin, and lactoferrin
Blood clotting agents
Anticoagulants and thrombolytic agents
Enzyme inhibitors
Interferons
Interleukins and "anti-interleukins"
Erythropoietin and other growth factors
Other therapeutic proteins
Monoclonal and catalytic antibodies
Recombinant antibodies
Therapeutic antibodies
Vaccines
Recombinant vaccines
Steroid biotransformations
Diagnostic enzymes
Enzyme tests
Biosensors
Immunoanalysis
Glycobiology
AGRICULTURE AND ENVIRONMENT
Animal breeding
Embryo transfer, cloned animals
Gene maps
Transgenic animals
Breeding, gene pharming and xenotransplantation
Plant breeding
Plant tissue surface culture
Plant cell suspension culture
Transgenic plants: methods
Transgenic plants: resistance
Transgenic plants: products
Aerobic wastewater treatment
Anaerobic wastewater and sludge treatment
Biological treatment of exhaust air
Biological soil treatment
Microbial leaching, biofilms, and biocorrosion
MEGATRENDS
The human genome
Functional analysis of the human genome
Pharmacogenomics, nutrigenomics
DNA assays
Gene therapy
Induced pluripotent stem cells (iPS)
Tissue engineering
Drug screening
High-throughput sequencing
Proteomics
DNA and protein arrays
Metabolic engineering
Synthetic biology
Systems biology
Bioinformatics: sequence and structural databases
Bioinformatics: functional analyses
Carbon sources (C-sources)
Biorefineries
SAFETY. ETHICS, IP
Safety in genetic engineering
Regulation of products derived from biotechnology
Ethical considerations and acceptance
Patents in biotechnology
International aspects of biotechnology

"...you should have it close at hand on your desktop as you read new articles. I would recommend this reference book...not only to clinical chemists, pathalogists, and medical technologists, but to anyone in medicine who wants to know how all the new therapeutic agents are made and appriciates the impact of biotechnology an medicine and society."
Clinical Chemistry

"...this book is a useful, interesting and colourful guide to modern biotechnology and genetic engineering. It will achieve its objective of providing students with an overview of the field presented in manageable portions and a clear and accessible manner, but it will also be a source of information, a useful reference and an interesting read for any researcher who is working across the traditional boundaries of chemistry, biology or biochemistry."
ChemBioChem

"This pocket guide can be recommended unreservedly for all students and researchers in natural and engineering sciences and medicine, but is also useful to readers with a general interest in biotechnology and genetic engineering. I can certainly agree with the final sentence of the book cover: A perfect introduction to the field - for professionals and students."
Angewandte Chemie IE

Erwähnung in: Process
"...provides a broad coverage of the relevant facts on products, methods and applications in biotechnology and genetic engineering...Instructive yet attractive color illustrations and a didactic approach throughout the book..."
Process worldwide

"Beginners and experts will like this wonderfully composed book."
Journal of Statistical Computation & Simulation

"In the wilderness of biotechnology, Schmid's "Pocket Guide to Biotechnology and Genetic Engineering" is a welcome addition that with all likelihood will find many friends. During the review process it has definitely found one! The book is excellently produced. The figures are as sharp as tacks and as informative as the text...The overall verdict is: useful and recommendable to students and biotechnologists alike."
Engineering in Life Sciences