Annual Reports on NMR Spectroscopy (eBook)

Cover 1
Contents 10
List of Contributors 6
Preface 8
Chapter 1. In Vivo Proton NMR Studies in Skeletal Musculature 14
1. Introduction 15
2. Structure and function of musculature 17
3. Characteristics of muscle tissue in MR imaging 23
4. Signal patterns in proton spectra of skeletal muscle 32
5. Quantification of spectral signals 41
6. Assessment of relaxation times, MT effects and diffusion characteristics 48
7. Studies on lipid metabolism in skeletal musculature 60
8. Characterization of muscle diseases 73
9. Muscle spectroscopy at higher field strength 79
10. Muscle spectroscopy using other nuclei than protons 81
Acknowledgements 83
References 84
Chapter 2. Quality Assessment of Horticultural Products by NMR 88
1. Introduction 89
2. NMR quality assurance of harvested fruit 91
3. NMR quality assurance of vegetables 114
4. NMR quality assurance of bulbs 117
5. The cellular origins of relaxation and diffusion contrast 118
6. On-line NMR sensor design 122
7. Concluding remarks 128
Acknowledgements 130
References 130
Chapter 3. Applications of NMR to Thermostable Proteins 134
1. Introduction 135
2. Thermostable proteins 135
3. Extremophilic organisms 136
4. Factors determining protein thermostability 137
5. NMR of thermostable proteins 138
References 162
Chapter 4. High Pressure NMR Studies on Lyotropic Lipid Mesophases and Model Biomembranes 176
1. Introduction 177
2. High-pressure NMR measurements 179
3. Pressure effects on the structure, dynamics and phase transitions of phospholipid vesicles 182
4. Conclusions 211
Acknowledgements 212
References 212
Chapter 5. Diffusion in a Closed Sphere 214
1. Introduction 214
2. The pulsed field gradient echo (PFGE) NMR experiment 215
3. The diffusion equation 217
4. The propagator for one-dimensional diffusion 218
5. Free diffusion 219
6. Diffusion in a box 221
7. Three-dimensional diffusion in a spherically symmetric system 222
8. Conclusion 229
References 229
Chapter 6. Automatic Analysis of NMR Spectra 230
1. Introduction 231
2. Spectral processing 232
3. Spectral analysis 234
4. Spectral prediction 240
5. Confirmation of structure 245
6. Requirements of multiple parallel synthesis or combinatorial chemistry 248
7. Structural elucidation 249
8. Conclusions 258
References 258
Index 266