Regulators and Effectors of Small GTPases, Part F: Ras Family I (eBook)

Cover 1
Title Page 4
Copyright Page 5
Table of Contents 6
Contributors to Volume 332 10
Preface 14
Volumes in Series 16
Section I: Protein Expression and Protein–Protein Interactions 36
Chapter 1. Mammalian Expression Vectors for Ras Family Proteins: Generation and Use of Expression Constructs to Analyze Ras Family Function 38
Chapter 2. Protein Transduction: Delivery of Tat–GTPase Fusion Proteins into Mammalian Cells 71
Chapter 3. Green Fluorescent Protein-Tagged Ras Proteins for Intracellular Localization 85
Chapter 4. Targeting Proteins to Membranes, Using Signal Sequences for Lipid Modifications 99
Chapter 5. Targeting Proteins to Specific Cellular Compartments to Optimize Physiological Activity 112
Chapter 6. Mapping Protein–Protein Interactions with Alka- line Phosphatase Fusion Proteins 123
Chapter 7. Assays of Human Postprenylation Processing En- zymes 138
Chapter 8. In Vivo Prenylation Analysis of Ras and Rho Pro- teins 150
Chapter 9. Ras Interaction with RalGDS Effector Targets 162
Chapter 10. RAS Interaction with RIN1 Effector Target 174
Chapter 11. Ras and Rap1 Interaction with AF-6 Effector Target 186
Section II: Screening Analyses 204
Chapter 12. Analysis of Protein Kinase Specificity by Peptide Libraries and Prediction of in Vivo Substrates 206
Chapter 13. Peptide Library Screening for Determination of SH2 or Phosphotyrosine-Binding Domain Sequences 218
Chapter 14. Expression Cloning of Farnesylated Proteins 230
Chapter 15. Expression Cloning to Identify Monomeric GTP- Binding Proteins by GTP Overlay 238
Chapter 16. Retrovirus cDNA Expression Library Screening for Oncogenes 246
Chapter 17. Identification of Ras-Regulated Genes by Representational Difference Analysis 256
Chapter 18. Differential Display Analysis of Gene Expression Altered by ras Oncogene 268
Chapter 19. cDNA Array Analyses of K-Ras-Induced Gene Transcription 280
Chapter 20. Ras Signaling Pathway for Analysis of Protein–Protein Interactions 295
Chapter 21. Isolation of Effector-Selective Ras Mutants by Yeast Two-Hybrid Screening 305
Chapter 22. Two-Hybrid Dual Bait System to Discriminate Specificity of Protein Interactions in Small GTPases 312
Chapter 23. Functional Proteomics Analysis of GTPase Signal- ing Networks 335
Section III: Analyses of Mitogen-Activated Protein Kinase Cascades 352
Chapter 24. Analyzing JNK and p38 Mitogen-Activated Protein Kinase Activity 354
Chapter 25. Phospho-Specific Mitogen-Activated Protein Kinase Antibodies for ERK, JNK, and p38 Activation 372
Chapter 26. Immunostaining for Activated Extracellular Signal-Regulated Kinases in Cells and Tissues 378
Chapter 27. Dominant Negative Mutants of Mitogen-Activated Protein Kinase Pathway 388
Chapter 28. Scaffold Protein Regulation of Mitogen-Activated Protein Kinase Cascade 403
Chapter 29. Bacterial Expression of Activated Mitogen-Activated Protein Kinases 422
Chapter 30. Steroid Receptor Fusion Proteins for Conditional Activation of Raf–MEK–ERK Signaling Pathway 436
Chapter 31. Pharmacologic Inhibitors of MKK1 and MKK2 452
Chapter 32. Analysis of Pharmacologic Inhibitors of Jun N-Ter- minal Kinases 467
Author Index 488
Subject Index 512